Key features and details | |
Cat. No. | MABL-1525 |
Name | Anti-Estradiol-17β mAbs |
Clone No. | AFD- E4-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, SPR, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for Estradiol-17β. Estradiol-17β is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles. |
Alternative Name | 17-beta-estradiol; E2; ethynyl estradiol; estradiol; oestradiol; ChEBI:16469 |
UniProt | |
Immunogen | This antibody was raised by immunizing a female balb/c mouse with immunogenic Estradiol- 17β-BSA conjugate. |
Application Notes | The scFv version of this antibody is capable of binding E2-7-BSA in an ELISA. A radioimmunoassay was performed using E2 standard and E2 with [H3] using the mouse version and the scFv version of this antibody. Finally the association and dissociation rate were determined using surface plasmon resonance on E2-7-BSA using both the mouse version and the scFv version of this antibody (Kobayashi et al, 2008; pmid:18824188) (Kobayashi et al, 2010; pmid:20047279). |
Antibody First Published | Kobayashi et al. Anti-estradiol-17beta single-chain Fv fragments: Generation, characterization, gene randomization, and optimized phage display. Steroids. 2008 Dec 22;73(14):1485-99. PMID:18824188 |
Note on publication | A single-chain Fv fragment (scFv) against estradiol-17beta (E(2)) was generated to begin the construction of a library of various mutated anti-steroid antibodies with an improved affinity and/or specificity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
