Anti-ESAT-6 mAbs

Cat. No.

MABL-1520

Name

Anti-ESAT-6 mAbs

Clone No.

AFD- EA-S3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Mycobacterium tuberculosis

Basic Information

Specificity

ESAT-6 is a potent T-cell antigen and   suggested as a diagnostic biomarker for TB infection.

Alternative Name

UniProt

B5TV89

Immunogen

BALB/c mice were immunised with ESAT-6 protein   and RNA extracted from the spleen to produce a scFv library. Eukaryotic   ribosome display was used to select EA-S3.

Application Notes

ELISA was used to confirm the specificity of   EA-S3 for ESAT-6; ELISA also determined the affinity constant of EA-S3 for   ESAT-6 as 3.74x10^8 M^-1 (Ahangarzadeh et al, 2017).

Antibody First   Published

Ahangarzadeh et al, 2018. Selection of   single-chain variable fragments specific for Mycobacterium tuberculosis   ESAT-6 antigen using ribosome display. Iran J Basic Med Sci. 2017   Mar;20(3):327-333 PMID:28392906

Note on publication

Describes the generation of EA-S3 from a   ribosome display library and its characterisation through ELISA.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.