Key features and details | |
Cat. No. | MABL-879 |
Name | Anti-CD31 mAbs |
Clone No. | AFD- JC70 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, WB, IF, IHC |
Species Reactivity | Rabbit, Human, Mouse |
Basic Information | |
Specificity | This antibody recognises CD31 (PECAM1) which is a member of the immunoglobulin superfamily and is expressed at various circulatng cells, such as neutrophils, monocytes, platelets as well as on endothelial cells. CD31 is a cell adhesion molecule and is involved in the leukocyte transendothelial migration during inflammation. |
Alternative Name | PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; JC/70A |
UniProt | P16284 |
Immunogen | This antibody was raised by immunising Balb/c mice with membrane preparation from a spleen affected by hairy cell leukaemia. |
Application Notes | This antibody recognises CD31 which is widely expressed on endothelial cells (Parums et al., 1990) and JC70 is a good marker of endothelial cells in paraffin sections. It may be used in the identification and characterisation of benign and malignant human vascular disorders (Parums et al., 1990). For instance, one group utilised this antibody to analyse formalin-fixed paraffin-embedded rat heart tissue in a study investigating relation between cisplatin and oxidative stress (Stojic et al., 2018). Similarly, another group labelled pancreatic tumour tissue samples against CD31 while studying factors influencing development of pancreatic cancer-associated thrombosis (Woei-A-Jin et al., 2016). Finally, Kandemir and colleagues (2012) stained formalin-fixed and paraffin-embedded samples of cutaneous Kaposi sarcoma in order to characterise its immunohistochemical profile. |
Antibody First Published | Parums et al. JC70：a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol. 1990 Sep;43(9)：752-7. PMID：2212067 |
Note on publication | This article describes generation and characterisation of JC70 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
