Cat. No.
MABL-1177
Application
IP, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B3-17
From
Recombinant Antibody
Specificity
This antibody is specific for human CEACAM1, and recognises an epitope in the extracellular domain.
Alternative Names
CD66a; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGPI
UniProt
P13688
Immunogen
This antibody was raised by immunising mice with recombinant human CEACAM1.
Application Notes
In flow cytometric analysis, this antibody has been used to detect CEACAM1 surface expression by human epithelial and endothelial cell lines, and microvesicles derived from these cells (Muturi et al, 2013), normal human bronchial epithelial (NHBE) cells (Klaile et al, 2013), CEACAM1-knockout human intestinal epithelial cells (Klaile et al, 2017) and human B-cell subpopulations (Khairnar et al, 2015; Seifert et al, 2015). It has also been used to detect CEACAM1 expression in ELISA analysis of transfected A549 epithelial cells (Singer et al, 2014). This antibody has been used in immunofluorescence analysis of NHBE cells (Klaile et al, 2013), as well as in the immunoprecipitation of CEACAM1 from NHBE cell culture supernatants (Klaile et al, 2013). Additionally, this antibody has been used in IHC staining of paraffin- embedded human lung tissue sections (Klaile et al, 2013).
Antibody First Published
Klaile et al. Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons. Respir Res. 2013 Aug 14;14:85 PMID:23941132
Note on publication
Describes the original use of this antibody, in FC, IF an IP analysis, to assess CEACAM1 expression by normal human bronchial epithelial (NHBE) cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
