Cat. No.
MABL-1178
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C5-1X/8
From
Recombinant Antibody
Specificity
This antibody is specific for human CEACAM1, and targets the targets the A1-B-domain.
Alternative Names
CD66a; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGPI
UniProt
P13688
Immunogen
This antibody was raised by immunising mice with recombinant human soluble CEACAM-1-Fc, produced in HEK293 cells.
Application Notes
This antibody has been used in ELISA analysis to detect and quantify soluble CEACAM1 in human bronchoalveolar lavage fluid samples (Klaile et al, 2013). This antibody has also been used in immunohistochemical staining of paraffin-embedded human brain tissue sections, revealing CEACAM1- positive B cells in brain infiltrates (Rovituso et al, 2016). Additionally, this antibody has been used in Western blot analysis of CEACAM1 expression in human intestinal epithelial cell lysates (Klaile et al, 2017). This antibody does not inhibit B cell aggregation in vitro (Rovituso et al, 2016).
Antibody First Published
Klaile et al. Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons. Respir Res. 2013 Aug 14;1416:0385 PMID:23941132
Note on publication
Describes the original use of this antibody, in a CEACAM1-specific sandwich ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
