Cat. No.
MABL-1169
Application
Activate, Block, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HBJ127
From
Recombinant Antibody
Specificity
HBJ127 binds to the CD98 heavy chain at an epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The antibody has not been shown to cross-react with other species. CD98 is expressed at high levels on monocytes and at very low level on peripheral blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a disulfide-linked and glycosylated type II integral membrane protein. This protein has roles in normal and neoplastic cell growth and is required for the function of light chain amino acid transporters. It is also involved in sodium-independent transport of large neitral amino acids and in guiding and targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, CD98 acts as an arginine/glutamine exchanger.
Alternative Names
4F2hc; HBJ 127; HBJ-127; CD98; GP125; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; Solute carrier family 3 member 2
UniProt
P08195
Immunogen
HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had been immunized with T24 hyman bladder cancer cells.
Application Notes
HBJ127 can inhibit lymphocyte activation and proliferation in vitro (Yagita and Hashimoto, 1986), as well as inhibit the growth of human bladder tumors (Yagita, 1986). The antibody also mediates the inhibition of DNA synthesis of Con A-stimulated lymphocytes at concentrations greater than 13 μg/ml, the inhibition of mAB 6-1-13-induced c-src expression and the inhibition of mAb 6-1-13-induced polykaryocyte formation. In addition, HBJ127 can enhance expression of the mannose receptor on the surface of monocytes derived from healthy humans. HBJ127 shows positive staining of murine L929 cells transiently expressing human CD98 heavy chain in IF studies. The antibody can also be used to detect CD98 heavy chain by ELISA.
Antibody First Published
Yagita et al. Monoclonal antibodies that inhibit activation and proliferation of lymphocytes. I. Expression of the antigen on monocytes and activated lymphocytes. J Immunol. 1986 Mar 15;136(6):2055-61. PMID:3950410
Note on publication
Describes the in vtro effect of HBJ127 and other mAbs on lymphocyte activation and proliferation, and gave evidence to suggdst that the HBJ127 antigen was important in the activation of lymphocytes and its subsequent progression through the cell cycle.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
