Cat. No.
MABL-1150
Application
Blocking, cell-mediated lympholysis assays., fixed lymphocyte reaction (MLR), FC, IF, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
YCATE55.9
From
Recombinant Antibody
Specificity
This antibody is specific for the alpha chain of canine CD8, and reacts with a rat cell line transfected with cDNA for canine CD8α (Gorman, 1994)
Alternative Names
LEU2; T-cell surface glycoprotein CD8 alpha chain; CD8a
UniProt
P33706
Immunogen
This antibody was prepared by immunizing rats with canine leucocytes, thymocytes or concanavalin A-activated lymphoblasts.
Application Notes
This antibody recognizes canine CD8α, and has been used in in IF and IHC studies to establish that CD8 expression is confined to a minority population of T cells, as is the case for other species (Cobbold, 1994). In a canine transplant model, administration of this antibody, in combination with another anti-CD8 antibody (YCATE60.3.9) causes a 90 per cent depletion of CD8+ T cells from the peripheral blood, but does not result in prolonged allograft survival (Watson, 1993). However, the administration of this antibody in combination with an anti-CD4 antibody is associated with an immunosuppressive effect, and an extended median graft survival time. This antibody blocks MHC class I dependant T-cell responses in vitro and in vivo.
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens： Summary of the First International Canine Leukocyte Antigen Workshop (CLAW) Tissue Antigens. 1994 Mar;43(3)：137-54. PMID：8091414
Note on publication
Describes the identification and characterisation of a panel of monoclonal antibodies that define canine homologues of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
