Cat. No.
MABL-1136
Application
crystallization, FACS, inhibition, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Ab10
From
Recombinant Antibody
Specificity
This antibody is specific for the large extracellular loop of human CD81.
Alternative Names
CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28 (Tspan-28)
UniProt
P60033
Immunogen
The original antibody was generated by immunization of Balb/c and Naval Medical Research Institute (NMRI) mice with CD81 extracellular loop 2 (ECL2) protein.
Application Notes
The scFv version of this antibody is based on an anti-CD81 clone whose binding specificity was determined via FACS that was successfully used in an inhibition assay against HCV (Ji et al., 2015; PMID： 25417967). The exact binding site of this antibody was determined via crystallization (Nelson et al., 2018; PMID： 29778602). The scFv version of this antibody was used successfully in FC (Susa et al., 2020; PMID： 32338599).
Antibody First Published
Nelson et al. Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies J Mol Biol. 2018 Jul 6;430(14)：2139-2152. doi： 10.1016/j.jmb.2018.05.018. Epub 2018 May 17 PMID：29778602
Note on publication
The original publication explores the development of antibodies targeting the host antigen CD81 to block hepatitis C viral entry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
