Cat. No.
MABL-1135
Application
functional, ICC/IF, IHC-P, IP, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Chimpanzee, Goat, Sheep
Clone No.
1D6
From
Recombinant Antibody
Specificity
This antibody is specific for human CD81, which is expressed on most tissues with the exception of red blood cells and platelets.
Alternative Names
Tetraspanin-28; TAPA-1; Target of the Antiproliferative Antibody 1; Tspan-28
UniProt
P60033
Immunogen
This antibody was produced by immunizing mice with 5A6-aggregated OCI-LY8 B cells.
Application Notes
This antibody is a strong inducer of homotypic adhesion in hematolymphoid cells (Levy, 1998). This antibody also has an anti-proliferative effect, and is able to induce growth inhibition of B cell lines. Triggering of CD81 by this antibody induces a significant upregulation of the constituitive release of TNF-α by JY cells, an Eptsein-Barr virus-positive B lymphoblastoid cell line (Altomonte, 1995).
Antibody First Published
Levy et al. CD81 (TAPA-1)： a molecule involved in signal transduction and cell adhesion in the immune system Annual review of immunology, 1998, Vol.16, pp.89-109 [Peer Reviewed Journal] PMID：9597125
Note on publication
Describes the original production of this antibody, and its use in characterising the CD81 antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
