Cat. No.
MABL-1134
Application
FACS, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
1.3.3.22
From
Recombinant Antibody
Specificity
1.3.3.22 antibody recognizes CD81. Human CD81 is a member of the tetraspanin family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. It is present on a wide variety of leukocytes.
Alternative Names
TSPAN-28; Tetraspanin-28; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; TAPA-1; TAPA1
UniProt
P60033
Immunogen
This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
Application Notes
Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas and thus 1.3.3.22 can be used as a research antibody in the studies on lymphoma therapeutics. This antibody is also recommended in the detection and analysis of CD81 via various methods. For instance, 1.3.3.22 antibody was used in the identification of CD81 in human arteries by immunohistochemistry (Rohlena et al., 2009). 1.3.3.22 was also successfully utilized during flow cytometric detection of CD81 on KM3 cells (Higginbottom et al., 2000).
Antibody First Published
Allander et al. Hepatitis C virus envelope protein E2 binds to CD81 of tamarins. Virology. 2000 Nov 25;277(2)：358-67. PMID：11080483
Note on publication
This article describes the application of the 1.3.3.22 antibody used in a flow cytometry study.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
