Cat. No.
MABL-1114
Application
FACS, IP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
14
From
Recombinant Antibody
Specificity
Clone 14 binds human CD8, which is a coreceptor for the T lymphocyte receptor (TCR). CD8 binds, as a heterodimer, to class 1 MHC molecules and facilitates antigen-specific activation of the adaptive immune response. CD8 mutations are associated with various immunodeficiencies and pathologies.
Alternative Names
Leu2; cluster of Differentiation 8; T lymphocyte differentiation antigen T8
UniProt
Immunogen
Murine antibodies were produced by immunising mice with human monocytes. Immunised cells were used to produce a hybridoma from which the antibodies were purified.
Application Notes
Clone 14 has been used to investigate the cell type-dependent expression of CD8 in nephrectomised rats by IHC methods (Xiao et al, 2014), and can further be used for detection of T cell population subsets.
Antibody First Published
Hogg et al. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional β2 integrins Mac-1 and LFA-1 J. Clin. Invest. 103:97–106 (1999). PMID:9884339
Note on publication
Describes a patient with leukocyte adhesion deficiency 1 (LAD-1) syndrome, characterised by lack of CD18 function. In this study, mAb clone 14 - produced in the authors's laboratory - was used as an anti-CD8 marker.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
