Cat. No.
MABL-1113
Application
Blocking, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2.4300000000000002
From
Recombinant Antibody
Specificity
This antibody is specific for the lyt-2 protein of mice (it is psecific to the Lyt2.2 variant which is expressed in the mouse strains BALB/c and C57BL/6 (B/6)). Lyt-2 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex.
Alternative Names
Lyt 2.2; T-cell surface glycoprotein CD8 alpha chain; Cd8a; T-cell surface glycoprotein Lyt-2; lyt-2; lyt2; CD_antigen: CD8a; 2.43.1
UniProt
P01731
Immunogen
This antibody was generated by immunizing a Lewis rat with i.v. 3 times at 2-wk intervals with 15 * 10^6 L3 cloned cytolytic T cells. Three days after the third immunization, the rat spleen cells were fused with either P3 or SP2/0 cells using polyethylene glycol.
Application Notes
This antibody was used for an immunofluorescence experiment on mouse thymocytes and mouse spleen cells (Sarmiento et al, 1980; pmid:6159417). Immunoprecipitation was preformed on NP- 40 extract of 126I surfae labeled L3 cells using this antibody (Sarmiento et al, 1980; pmid:6159417). This antibody was used to try and block the cytolytic activity from L3 against P-185. It only blocks the receptor- mediated but not lectin mediated cytolysis by L3 (Dialynas et al, 1981; pmid:6788878). Flow cytometry was preformed with this antibody on washed cells from the lymph node, spleen, thymus and peripheral blood from mice (Rodriguez and Sriram, 1988; pmid:2966203). This antibody was used to stain mouse spleen cells for use in flow cytometry (Mielke et al, 1988; pmid:2969373).
Antibody First Published
Sarmiento et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement J Immunol. 1980 Dec;125(6):2665-72. PMID:6159417
Note on publication
In the paper five rat monoclonal antibodies were derived that express specificities for determinants present on the molecular complex bearing the Lyt 2 antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
