Cat. No.
MABL-1115
Application
Blocking, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
51.1
From
Recombinant Antibody
Specificity
The antibody is specific for CD8.
Alternative Names
CD8a; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
UniProt
P01732
Immunogen
The original antibody was generated by immunizing mice with CD1d.
Application Notes
The antibody could block HLA-A2.1-mediated binding to CD8α(+) expressed on CHO cells (Norment et al, 1988; PMID: 3263576). The antibody could also block TLA-mediated binding (Teitell et al., 1991; PMID: 1834760). The antibody was used to localize CD8α to the cell periphery on monocytes using confocal microscopy of permeabilized PBMC. The antibody could detect CD8α by flow cytometry on CD14hi monocytes from human peripheral blood (Gibbings et al. 2007; PMID: 17678538).
Antibody First Published
Martin et al. Cell-cell adhesion mediated by CD8 and MHC class I moleculesEpitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32 J Immunol. 1984 Feb;132(2):759-65. PMID:6197474
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
