Cat. No.
MABL-1112
Application
IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
HM79-16
From
Recombinant Antibody
Specificity
Reacts with an extracellular epitope of CD79b.
Alternative Names
Ig-beta; AGM6; B29; IGB; CD79b molecule;
UniProt
P15530
Immunogen
HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Application Notes
A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).
Antibody First Published
Mariko Koyama et al. CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated µ heavy chain International Immunology, Vol. 9, No. 11, pp. 1767–1772 1997 PMID:9418137
Note on publication
Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
