Cat. No.
MABL-1106
Application
IP, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Chicken, Cow, Guinea Pig, Horse, Opossum, Pig, Primate, Rabbit, Rat, Human, Mouse
Clone No.
HM57
From
Recombinant Antibody
Specificity
This antibody recognises CD79a which is involved in initiation of the signal transduction cascade activated by binding of antigen to the B-cell receptor. CD79a is used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissue sections.
Alternative Names
MB-1; B-cell antigen receptor complex-associated protein alpha chain; Ig- alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM- associated protein; MB-1 membrane glycoprotein
UniProt
P11912
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide G-T-Y-Q-D-V-G- S-L-N-I-A-D-V-Q, representing amino acids 202-216 of the human mb-1 protein and conjugated to thyroglobulin.
Application Notes
CD79a is strongly expressed in various B-cell-derived conditions and thus this antibody is useful during identification and investigation of such diseases (via IF, IHC) (Mason et al., 1991). One group utilised it for immunofluorescent labelling of CD79a containing cells in bone marrow samples of pre-B lymphocyte acute leukemia patients (Mason et al., 1991). HM57 antibody can also be used for IP as it was very efficient in immunoprecipitating CD79a from NP-40 cells lysates (Mason et al., 1995). Finally, this antibody has been widely applied in veterinary research and proved successfull in recognising CD79a in various species, from chicken to dog (Doring et al., 2018).
Antibody First Published
van Noesel et al. The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8. PMID:2033258
Note on publication
This article describes generation and application of HM57 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
