Cat. No.
MABL-1107
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JCB117
From
Recombinant Antibody
Specificity
This antibody recognises CD79a which is involved in initiation of the signal transduction cascade activated by binding of antigen to the B-cell receptor. CD79a is used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissue sections.
Alternative Names
MB-1; B-cell antigen receptor complex-associated protein alpha chain; Ig- alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM- associated protein; MB-1 membrane glycoprotein
UniProt
P11912
Immunogen
This antibody was raised by immunising mice with a recombinanat mb-1 protein followed by the preparation of a murine hybridoma.
Application Notes
This antibody reacts very well with B-cells during immunohistochemical labelling of paraffin-embedded tissue samples. It detected most B-cell neoplasms tagging B-cells at various stages of maturation (Mason et al., 1995). Consequently, it may be used as a reliable marker in the detection and investigation of different B-cell conditions. For instance, it labels precursor B-cell acute lymphoblastic leukemia samples what makes it the most efficient marker of this disorder in paraffin-embedded tissue (Mason et al., 1995). Furthermore, this antibody may be useful in staining and identification of plasma cell myeloma and acute promyelocytic leukemia. For example, JCB117 antibody was utilised in the characterisation of multiple myeloma and it showed very strong immunostaining of the neoplastic cells (Requena et al., 2003).
Antibody First Published
Mason et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86(4):1453-9. PMID:7632952
Note on publication
This article describes generation of JCB117 antibody and its application in immunohistochemistry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
