Cat. No.
MABL-1105
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Chicken, Guinea Pig, Horse, Opossum, Pig, Primate, Rabbit, Rat, Human, Mouse
Clone No.
HM47
From
Recombinant Antibody
Specificity
This antibody recognises CD79a which is involved in initiation of the signal transduction cascade activated by binding of antigen to the B-cell receptor. CD79a is used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissue sections.
Alternative Names
MB-1;B-cell antigen receptor complex-associated protein alpha chain; Ig- alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM- associated protein; MB-1 membrane glycoprotein;
UniProt
P11912
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide G-T-Y-Q-D-V-G- S-L-N-I-A-D-V-Q, representing amino acids 202-216 of the human mb-1 protein and conjugated to thyroglobulin.
Application Notes
IP: HM47 antibody was successfully used in immunoprecipitation of samples coming from NP-40 lysates of surface-labelled Daudi cells what confirmed participation of the mb-1 gene product in mIgM-associated heterodimer; subsequently a reprecipitation was performed as a confirmatory step (van Noesel et al., 1991). IHC: This antibody presented strong selective staining of B cell follicles in sections of lymphoid tissue (Mason et al., 1991). HM47 showed also high specificity in staining bone marrow samples with acute promyelocytic leukemia (APL) (stained 86% APL cases and only 10% of non-APL samples) (Bhargava et al., 2007).
Antibody First Published
van Noesel et al. The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8. PMID:2033258
Note on publication
This article describes generation and application of HM47 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
