Cat. No.
MABL-1070
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
FA-11
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD68, known also as macrosialin (MS). CD68 is a 110 kDa, heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the lysosomal/endosomal-associated membrane glycoproteins (LAMP). CD68 is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several acute myeloid leukemia studies.
Alternative Names
Macrosialin; FA/11; FA11 antigen
UniProt
P31996
Immunogen
This antibody was raised by immunising rats (AO x Lou strain) with murine macrosialin (MS).
Application Notes
When it was first generated, this antibody was used in binding assays, immunoprecipitation (IP) and immunofluorescence (IF) to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes (Smith et al., 1987). Subsequently, this antibody has been utilised in numerous FACS, IP, IHC, ICC/IF, WB applications, for instance, to delineate the roles of mouse and human CD68 as macrophage receptors for oxidised low density lipoprotein (Ramprasad et al., 1996), to indicate that mice unresponsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) are unexpectedly resistant to cutaneous Leishmania major infection (Scott et al., 2000), to characterise the macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice (Lang et al., 2000), and to examine the role of Foxp3(+) Tregs in Alzheimer's disease (Baruch et al., 2015).
Antibody First Published
Smith et al. Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. J Cell Sci. 1987 Feb;87 ( Pt 1):113-9. PMID:3312248
Note on publication
Describes the original generation of this antibody and its subsequent applications in binding assays, immunoprecipitation and immunofluorescence to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
