Cat. No.
MABL-1060
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CBL 08 6850
From
Recombinant Antibody
Specificity
This antibody is specific for CD62P.
Alternative Names
AK-6; SELP; GMP-140; LECAM3; PADGEM; P-selectin; Granule membrane protein 140; CD62 antigen-like family member P; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane protein
UniProt
P16109
Immunogen
The original antibody was raised by immunizing BALB/c mice with an ~100-200-kDa human platelet glycoprotein fraction prepared by gel filtration of concanavalin A-purified platelet membrane glycoproteins.
Application Notes
This antibody was used for immunohistochemistry on normal dermal endothelium (Fleming et al., 2008; PMID:18197262, Foubert et al., 2015; PMID:24069908), and on nasal polyp tissue and associated inferior turbinate tissue (Könnecke et al., 2014; PMID:24995349). The original antibody was used for immunoprecipitation on platelet membrane glycoproteins. This antibody was also used for a western blot on cultured human umbilical vein endothelial cells (Skinner et al., 1989; PMID:2480118). This antibody was used for immunofluorescence on human umbilical vein endothelial cells (Mourik et al., 2013; PMID:24010820).
Antibody First Published
Skinner et al. Characterization of human platelet GMP-140 as a heparin- binding protein Biochem Biophys Res Commun. 1989 Nov 15;164(3):1373-9. PMID:2480118
Note on publication
The original paper studies the interaction of Human platelet GMP-140 and heparin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
