Cat. No.
MABL-1059
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-85
From
Recombinant Antibody
Specificity
OX-12 binds to rat antibody kappa light chains - has preference for IgKa allotype.
Alternative Names
L-selectin; LECAM-1; Ly-22; MEL-14; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen
UniProt
P30836
Immunogen
This antibody was prepared by immunizing 8 –12-week-old BALB/c mice with purified rat CD62L-CD4
Application Notes
This antibody binds to CD62L expressed on rat lymphocytes, and has been used to show that recombinant soluble rat CD62L-CD4, to which it also binds, is likely to be correctly folded (Nicholson, 1998). This antibody also labels, and can therefore be used to fractionate, the subpopulation of rat CD4+CD8- thymocytes that expresess L-selectin (Seddon, 1996). Cells with this phenotype are able to protect rats from diabetes upon adoptive transfer, as well as providing B cells with help for secondary antibody responses and mediating local graft-versus-host reactions.
Antibody First Published
Seddon et al. CD4+CD8- thymocytes that express L-selectin protect rats from diabetes upon adoptive transfer European Journal of Immunology, Volume 26, Issue 11, 1996, Pages 2702-2708 PMID:8921958
Note on publication
Describes the original use of this antibody to isolate the subpopulation of rat CD4+CD8- thymocytes that express L-selectin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
