Cat. No.
MABL-1056
Application
Blocking, IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human, Chimpanzee, Bovine
Clone No.
DREG-56
From
Recombinant Antibody
Specificity
This antibody is specific for human L-selectin, a homing receptor selectively expressed on lymphocytes, which allows their entry into secondary lymphoid tissues via high endothelial venules.
Alternative Names
L-selectin; LECAM-1; Ly-22; MEL-14; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen
UniProt
P14151
Immunogen
This antibody was produced by immunizing mice with the concentrated supernatant generated after centrifugation of human peripheral blood leukocytes.
Application Notes
This antibody has been used in WB, IP and binding assays to define a lymphocyte homing receptor for PLN HEVs (Kishimoto, 1990). In vitro, this antibody has been shown to specifically inhibit over 90% of binding of human lymphocytes to HEVs within frozen sections of peripheral, but not mucosal, lymphoid tissue (Kishimoto, 1990). This antibody has also been used in labelling studies to demonstate that the trophectoderm of implantation-competent human embryos expresses L-selectin (Genbacev, 2003).
Antibody First Published
Kishimoto et al. Identification of a Human Peripheral Lymph Node Homing Receptor: A Rapidly Down-Regulated Adhesion Molecule Proceedings of the National Academy of Sciences of the United States of America, 1 March 1990, Vol.87(6), pp.2244-2248 PMID:2179952
Note on publication
Describes the original use of this antibody, along with others, to define a lymphocyte homing receptor (L-selectin).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
