Cat. No.
MABL-1055
Application
in vitro, inhibition, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CY1788
From
Recombinant Antibody
Specificity
This antibody is specific for E-selectin. E-selectin is a cell surface receptor that mediates the binding of neutrophils, monocytes, and some types of memory T cells to the endothelial cells that line blood vessels.
Alternative Names
E-selectin; ELAM-1; LECAM-2; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; CY 1788; CY- 1788; humanized CY1787; hu CY1787; huCY1787; CY1788 (VHF/VLA); CY1788Vu; CY1788VB
UniProt
P16581
Immunogen
The original antibody was generated by replacing the murine variable regions of the monoclonal antibody CY1787 with human sequences.
Application Notes
The original version of this antibody (human IgG4) was generated, and its binding efficiency to a soluble form of E-selectin was compared to that of the parental mouse antibody via competitive ELISA. It demonstrated 65% of the binding efficiency of the parental clone. Additionally, this antibody inhibited the in vitro binding of human neutrophils to activated human umbilical vein endothelial cells (HUVECs) in vitro as effectively as the parental antibody (WO1996040942A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
