Cat. No.
MABL-1057
Application
IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Guinea Pig, Rat, Human, Mouse
Clone No.
LAM1-116
From
Recombinant Antibody
Specificity
This antibody is specific for the natural ligand binding domain of L-selectin.
Alternative Names
SELL; L-selectin; LAM-1; LECAM1; Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; Lymph node homing receptor; CD62 antigen-like family member L; Leukocyte-endothelial cell adhesion molecule 1
UniProt
P14151
Immunogen
This original antibody was raised by immunizing a L-selectin deficient mouse with L-selectin.
Application Notes
The original antibody was used for flow cytometry on Holstein calve and sheep leukocytes (Leid et al., 2001; PMID:11290767), on EDTA-anticoagulated guinea pig blood (Fryer et al., 1997; PMID:9109449), an d on plasma samples from rats (Yan et al., 2000; PMID:10803628). This antibody was used for immunoprecipitation of L-selectin from bovine lymphocyte lysates (Leid et al., 2001; PMID:11290767), and on RBL cells (Haribabu et al., 1997; PMID:9153259). This antibody was used to test the effect of the antibody on NF-kappa B activation. It was shown that this antibody could indeed activate NF-kappa B in neutrophils (Turutin et al., 2003; PMID:12586371). This antibody was used for immunohistochemistry on a rat endometriosis model and on tissues of human endometriosis. This antibody was able to show the localization of the protein (Odagiri et al., 2007; PMID:17482182). This antibody was used for immunofluorescence on RBL cells where it showed staining like expected (Haribabu et al., 1997; PMID:9153259).
Antibody First Published
Steeber et al. Ligation of L-selectin through conserved regions within the lectin domain activates signal transduction pathways and integrin function in human, mouse, and rat leukocytes J Immunol. 1997 Jul 15;159(2):952-63. PMID:9218616
Note on publication
Since ligation of L-selectin through its ligand recognition region may mimic physiologic ligand binding, the original paper generated a new panel of mAbs that engaged a conserved ligand-binding region within the lectin domains of human, mouse, and rat L-selectin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
