Cat. No.
MABL-1016
Application
IP, Block, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
OX-78
From
Recombinant Antibody
Specificity
OX-78 binds specifically to CD48 targets and labels mouse thymocytes, B and T cells and macrophages. CD48 is a CD2 ligand and may be involved in the facilitation of interactions between activated lymphocytes. This protein is also likely to be involved in T cell activation.
Alternative Names
BCM1; BCM1 surface antigen; BLAST1; BLAST-1; HM48-1; MRC OX-45 surface antigen; MRC OX45 surface antigen; OX45 surface antigen; OX-45 surface antigen; SLAM family member 2; sgp60; sgp-60; OX78; MRC OX-78; MRC OX78
UniProt
P18181
Immunogen
OX-78 was prepared by immunising rats against mouse CD48.
Application Notes
This antibody binds CD48, and has been used in immunoprecipitation and blocking studies to identify and characterise CD48, the ligand for murine CD2 (Kato, 1992). This antibody is able to block the binding of a chimeric molecule comprised of the extracellular domain of mouse CD2 and human IgG1 Fc to T cell lines at the ligand site.
Antibody First Published
Kato et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med. 1992 Nov 1;176(5)：1241-9. PMID：1383383
Note on publication
Describes the original use of this antibody to isolate the subpopulation of rat CD4+CD8- thymocytes that express L-selectin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
