Cat. No.
MABL-1017
Application
IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
16B4
From
Recombinant Antibody
Specificity
This antibody is specific for the α2 integrin subunit, and recognises an epitope outside the von Willebrand factor A-type, or collagen-binding domain. Integrins are heterodimeric integral membrane glycoproteins, which are expressed by a range of cells, including T cells, NK cells, fibroblasts and platelets, where they mediate cell adhesion.
Alternative Names
Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; alpha 2 subunit of VLA-2 receptor; BR; CD49B; human platelet alloantigen system 5; ITGA2; platelet antigen Br; platelet glycoprotein GPIa; very late activation protein 2 receptor, alpha-2 subunit
UniProt
P17301
Immunogen
Application Notes
This antibody has been used in flow cytometric analysis to investigate the surface expression of α2 integrin by cultured human endothelial cells (Sincock et al, 1999), and in immunoprecipitation analysis to assess the interaction between PETA-3 and integrins on the surface of endothelial cells (Sincock et al, 1999). Alexa Fluor 647-conjugated 16B4 has been used to evaluate the expression of integrin α2β1 on the surface of platelets by confocal imaging (Poulter et al, 2017). This antibody has been employed to detect integrin α2β1 expression through in-cell Western blotting analysis in primary fibroblasts (Suarez et al, 2014). This antibody has also been used to coat polystyrene beads as part of a microsphere-based cellular adhesion assay; human osteosarcoma cells transfected with the integrin a2 subunit displayed high levels of interaction with beads coated in this anti-α2 integrin antibody (Connors & Heino, 2004).
Antibody First Published
Sincock et al. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function Journal of Cell Science 112, 833-844 (1999) PMID：10036233
Note on publication
Describes the original use of this antibody in flow cytometric and immunoprecipitation analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
