Cat. No.
MABL-1015
Application
Blocking, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-45
From
Recombinant Antibody
Specificity
OX-45 binds specifically to CD48 on rat thymocytes, T and B cells and endothelium but not LFA.3. Directly competes with CD46 for binding the target epitope. CD48 is a CD2 ligand and is expressed on the surface of all hematopoietic cells and endothelium. May be involved in the facilitation of interactions between activated lymphocytes. Likely to be involved in T cell activation.
Alternative Names
BCM1; BCM1 surface antigen; BLAST1; BLAST-1; HM48-1; MRC OX-45 surface antigen; MRC OX45 surface antigen; OX45 surface antigen; OX-45 surface antigen; SLAM family member 2; SLAMF2; sgp60; sgp-60; signalling lymphocytic activation molecule 2 OX45; MRC OX-4
UniProt
P10252
Immunogen
OX-45 was prepared by immunizing BALB/c mice with rat T blasts prepared by stimulating purified T helper (TH) cells with allogeneic irradiated rat spleen cells.
Application Notes
Describes the use of OX-45 in blocking the primary mixed lymphocyte response (MLR)and the secondary response of T lymphocytes to the soluble antigen DNP-BGG. It also showed that OX- 45 did not have any effect on the cytotoxic activity of specific (CTL) or non-specific (NK) killer cells or on proliferative responses stimulated by lectins or oxidative mitogenesis.
Antibody First Published
Arvieux et al. Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells. Immunology 58：337 1986 PMID：2942461
Note on publication
Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
