Cat. No.
MABL-932
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
W3/25
From
Recombinant Antibody
Specificity
Recognises a 50kDa Cell surface glycoprotein expressed by helper T cells - found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages.
Alternative Names
T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu- 3; W3/25 antigen; CD4
UniProt
P05540
Immunogen
Membraneous glycoproteins from rat thymocytes.
Application Notes
This antibody binds to rat CD4 and is able to suppress IL2 production in the Mixed Lymphocyte Reaction Assay.
Antibody First Published
Williams AF, Galfrè G, Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663-73.PMID:303545
Note on publication
Describes the generation of the antibody, shows it recognised rat thymocytes, and analysed by immunoblotting and fluorescence activated cell sorter.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
