Cat. No.
MABL-933
Application
FC, IHC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
YKIX302.9
From
Recombinant Antibody
Specificity
This antibody is specific for the canine CD4 cell surface antigen, which, in contrast to other mammals, is expressed by neutrophils as well as lymphocyte subsets.
Alternative Names
Leu3/T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
UniProt
P33705
Immunogen
This antibody was prepared by immunizing rats with concanavilin A activated T-cell blasts
Application Notes
This antibody recognizes canine CD4, which binds MHC II, and has been shown to block MHC II-dependent T cell responses in vivo and in vitro. In a canine transplant model, administration of this antibody has been demonstrated to partially deplete circulating T lymphocytes, but alone was not sufficient to prolong allograft survival (Watson, 1993). This antibody is one of a panel of anti-canine monoclonal antibodies can be used in the evaluation of leukemic status in dogs. This antibody has been used in IF and IP analysis to identify the canine homologue of human CD4 (Cobbold, 1994).
Antibody First Published
Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW) Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091415
Note on publication
Describes the identification and characterisation of a panel of monoclonal antibodies that define canine homologues of human CD antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
