Cat. No.
MABL-931
Application
IP, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-38
From
Recombinant Antibody
Specificity
This antibody binds rat CD4 cell surface antigen. CD4 is a transmembrane glycoprotein expressed on T helper cells, thymocytes, monocytes and macrophages. It plays an important role in the response of T helper cells to antigen. This antibody binds an identical or adjacent epitope to that bound by W3/25 antibody, and competes with mouse anti-rat CD4 antibody W3/25 for binding rat CD4.
Alternative Names
W3/25 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3;
UniProt
P05540
Immunogen
The original antibody was generated by immunizing mice with rat lymphocytes or rat thymocyte glycoproteins.
Application Notes
This antibody can be used to identify CD4 antigen present on T cells, thymocytes, monocytes etc. using flow cytometry. This antibody was also able to immunoprecipitate detergent extracts of iodine 125 labeled thymocytes (PMID: 3159821). This antibody can be used for the in vitro depletion of T helper cells from slice cultures in studies looking for myelin loss in multiple sclerosis, the observed disruption being abrogated in these experimental conditions by T-cell depletion (PMID: 25500111). This antibody was tested for its ability to prolong the survival of heterotopic vascularized rat heart allografts transplanted across major histocompatibility barriers. Nine of 12 treated rats have maintained heterotopic abdominal heart allografts for greater than 175 days (PMID: 1976282). This antibody was used in a study to demonstrate that monoclonal antibody-induced depletion of CD4 helper/inducer T lymphocytes before transplantation of a fully mismatched heart allograft allows permanent engraftment in rats without further immunosuppression (PMID: 1977898).
Antibody First Published
Jefferies et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985 Jul 1;162(1):117-27. PMID:3159821
Note on publication
Describes the generation of this antibody and its use in the characterization of the CD4 (W3/25) antigen present on rat peritoneal macrophages.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
