Cat. No.
MABL-930
Application
functional, IP, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-35
From
Recombinant Antibody
Specificity
OX-35 shows specificity towards rat CD4 antigen. W3/25 and OX-36 bind to different epitopes on CD4 antigen. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction and is involved in regulation of T-cell activation.
Alternative Names
Leu3/T4; L3T4; T4; Leu3; Leu-3; Ly4; Ly-4; W3/25 antigen; OX35; MRC OX-35; MRC OX35
UniProt
P05540
Immunogen
OX-35 was prepared by immunizing mice with T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen.
Application Notes
This antibody is able to label cells expressing CD4 on their surface and can precipitate CD4 from detergent extracts of radioactively labelled thymocytes.
Antibody First Published
Robinson et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3 Immunology 1986 57 239-247 PMID:3512425
Note on publication
Describes the original use of this antibody to detect SIRP alpha in labelling, immunoperoxidase and immunofluorescence studies to investigate macrophage heterogeneity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
