Cat. No.
MABL-927
Application
neutralising, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
DB81
From
Recombinant Antibody
Specificity
This antibody is specific for human CD4, and recognises an epitope in the vicinity of a 13- amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.
Alternative Names
Lu/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3
UniProt
P01730
Immunogen
This antibody was raised by immunising BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).
Application Notes
This antibody preferentially binds to a complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance analysis has shown that the binding affinity of this antibody to the CD4- gp120 complex is almost one order of magnitude higher than its binding to CD4 alone (Burastero et al, 2011), while ELISA analysis has confirmed that the epitope recognised by this antibody does not involve gp120 (Burastero et al, 2011). This antibody, through a post-binding mechanism, inhibits the cell fusion and replication of a range of HIV-1 strains (Burastero et al, 2011). It does not exert inhibitory effects on T-cell activation in vitro, a useful property for clinical use.
Antibody First Published
Burastero et al. Broad-Spectrum Inhibition of HIV-1 by a Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 PLoS One. 2011; 6(7): e22081. PMID:21818294
Note on publication
Describes the original generation of this antibody, and characterisation of its binding specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
