Cat. No.
MABL-928
Application
functional assay, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
GK1.5
From
Recombinant Antibody
Specificity
This antibody is specific for CD4. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Alternative Names
T-cell surface glycoprotein CD4; Cd4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3
UniProt
P06332
Immunogen
The antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll- Hypaque-purified cells from the cloned CTL line 243/2.5
Application Notes
The effect of this antibody on the PFC response was measured by treating spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170).Immunofluorescence was preformed preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). An flow cytometry experiment was preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). The effect of this antibody on antigen-specific release of IL2 by mouse T cell hydridomas (Wilde et al, 1983; pmid:6195255). ELISA was preformed with this antibody on calf fetal serum (Wilde et al, 1983; pmid:6195255). The effect of the antibody on antigen-specific binding by T cell hybridoma AODH-3.4 was determined (Wilde et al, 1983; pmid:6195255). ELISA was preformed on murine CD4 using this antibody (Freise et al, 2017; pmid:27966069). The biodistribution of this antibody in mice was determined (Freise et al, 2017; pmid:27966069). Flow cytometry was preformed on blood, Thymus, lymph nodes, and spleen cells from mice with this antibody (Freise et al, 2017; pmid:27966069). Mouse C57BL/6 splenocytes were used in a proliferation assay with this antibody (Freise et al, 2017; pmid:27966069). Mouse Blood, spleen, and lymph node lymphocyte were used for flow cytometry using this antibody (Rice et al, 1995; pmid:7539027). Multiple organs from mice were used for immunohistochemistry using this antibody (Rice et al, 1995; pmid:7539027). Flow cytomic detection was used to detect T cell depletion using this antibody (Rashid et al, 1992; pmid:1538121).
Antibody First Published
Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule J Immunol. 1983 Nov;131(5):2445-51.PMID:6415170
Note on publication
In the paper the antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll-Hypaque-purified cells from the cloned CTL line 243/2.5.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
