Cat. No.
MABL-919
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Pig
Clone No.
898H2-6-15
From
Recombinant Antibody
Specificity
Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.
Alternative Names
Leu4/T3; Cluster of Differentiation 3 epsilon chain; T cell glycoprotein CD3 epsilon chain
UniProt
Q7YRN2
Immunogen
Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
Application Notes
The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor- associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.
Antibody First Published
Huang et al. Characterization of a monoclonal anti-porcine CD3 antibody Xenotransplantation 1999:5: 201-212 PMID:10503787
Note on publication
Describes the identification and qualitative characterisation of murine anti-pig CD3e antibodies from hybridoma. These were shown to be specific for pig T lymphocytes, as opposed to human and baboon peripheral blood cells, and mouse spleen cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
