Cat. No.
MABL-920
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Chicken, Cow, Guinea Pig, Horse, Pig, Primate, Rabbit, Rat, Human
Clone No.
PC3/188A
From
Recombinant Antibody
Specificity
This antibody recognises CD3 epsilon which is a part of the TCR-CD3 complex present on all mature T-lymphocyte cells and is crucial in the adaptive immunity.
Alternative Names
Leu4/T3; T-cell surface glycoprotein CD3 epsilon chain; -cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3 epsilon; CD3e antigen; TCRE
UniProt
P07766
Immunogen
This antibody was raised by immunising mice with a peptide sequence (156-168) from the intracytoplasmic portion of the epsilon-chain of CD3.
Application Notes
This anti-CD3 has been used for the identification of normal and malignant T lymphocytes in paraffin embedded or frozen tissue sections. For instance, one group labelled paraffin- embedded pancreatic sections with PC3/188A antibody to identify T-lymphocytes while investigating the role of the oligodeoxynucleotide IMT504 on diabetes in rats (Bianchi et al., 2012). Another group also used this antibody to identify T lymphocytes by staining rat brain tissue samples against CD3 in a study investigating the mechanisms of neuroinflammation (Doursout et al., 2013). Finally, Roballo and Bushman (2019) utilised PC3/188A antibody as a marker of T cells during immunohistochemical analysis of frozen sciatic nerve sections.
Antibody First Published
Jones et al. Detection of T and B cells in many animal species using cross- reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35. PMID:8515069
Note on publication
This article describes generation and characterisation of PC3/188A antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
