Cat. No.
MABL-909
Application
crystallography, therapeutic, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MU1053
From
Recombinant Antibody
Specificity
MU1053 binds an epitope close to the C-terminal region of CD38. The residues composing this epitope are: N120, K121, F135, Q139, M142, D202, V203, H205, Q236, T237, E239, W241, Q272, S274, K276, I278, F284, C287, V288, P291, E292, S294, C296.
Alternative Names
Cluster of Differentiation 38; ADP-Ribosyl Cyclase 1; ADPRC1; ADPRC 1; 2-Phospho-Cyclic-ADP-Ribose Transferase; Cyclic ADP-Ribose Hydrolase 1; 2-Phospho-ADP-Ribosyl Cyclase; NAD(+) Nucleosidase; ADP-Ribose Transferase; Ecto-Nicotinamide Adenine Dinucleoti
UniProt
P28907
Immunogen
The C-terminal domain of human CD38 (residue 45–300) was expressed and produced in a yeast expression system. The purified recombinant protein was used as an antigen to immunize llamas. Phage display technology was used to obtain positive clones against CD38.
Application Notes
The nanobody MU1053 has been crystallised with CD38, and has been used to target molecules to CD38. Tagging the nanobody with fluorescent proteins allows the quantification of CD38 expression by flow cytometry, which can be used to identify malignant multiple myeloma cells, which have higher CD38 expression. Further, a form of MU1053 conjugated to a toxin has been developed as a potential therapeutic agent to selectively kill CD38+ cells.
Antibody First Published
Li et al. Immuno-targeting the multifunctional CD38 using nanobody. Sci Rep. 2016 Jun 2;6:27057 PMID:27251575
Note on publication
Describes increased CD38 expression in malignant cells and its potential as a drug target for cancer therapy.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
