Cat. No.
MABL-896
Application
IA assay, in vivo, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Chimpanzee, Baboon, Rhesus monkey, Human, Cynomolgus Monkey, Mouse
Clone No.
HB-8592
From
Recombinant Antibody
Specificity
This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11; it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chimpanzee, Baboon, Rhesus, and Cynomolgus monkey CR1. The scFv version of this antibody reacted with Mouse CR1.
Alternative Names
CR1; C3BR; C4BR; HB8592; HB 8592; MoAb 543
UniProt
P17927
Immunogen
The original antibody was generated by immunizing mice with purified CR1 from human erythrocytes.
Application Notes
This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia- derived CD4+ T cell lines by flow cytometry (Hart et al., 1992; PMID: 1606734) (Yaskanin and Waxman, 1995; PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992; PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997; PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008; PMID: 18385459).
Antibody First Published
Hart et al. Peritoneal macrophages during peritonitis. Phenotypic studies. Clin Exp Immunol. 1992 Jun;88(3):484-91. doi: 10.1111/j.1365-2249.1992.tb06476.x. PMID:1606734
Note on publication
Macrophage phenolypicprofile was compared with that of normal peripheral blood monocytes, anticipating that the phenotypic profile reflects the functional activity of the cell.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
