Cat. No.
MABL-897
Application
Blocking, IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
185-1G2 (B467)
From
Recombinant Antibody
Specificity
This antibody is specific against CD36. The antibody recognises an epitope between amino acids 155 - 183. CD36 is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. It acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry as well as mediates uptake of M. fortuitum, E. coli and S. aureus.
Alternative Names
SRB1; Scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CLA-1; CD36 antigen-like 1; Collagen type I receptor, thrombospondin receptor-like 1; SR-BI
UniProt
Q8WTV0
Immunogen
This antibody was raised by immunizing mice with stimulated human leukocytes.
Application Notes
This antibody is recommended for the detection and analysis of CD36 via various methods. For instance, it was used to stain ovarian cancer tissue samples via immunohistochemistry, demonstrating that CD36 was expressed in the cytoplasm of cancer cells while stromal cells were negative (Têtu et al., 2008). Furthermore, 185-1G2 antibody was shown to precipitate CD36 from a platelate lysate sample (Miao et al., 2001). Finally, this antibody has also blocking properties against CD36 and has been widely used to inhibit this receptor (Short et al., 2005).
Antibody First Published
Miao et al. CD36 associates with CD9 and integrins on human blood platelets. Blood. 2001 Mar 15;97(6):1689-96. PMID:11238109
Note on publication
The article describes the use of the 185-1G2 antibody in immunoprecipitations.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
