Cat. No.
MABL-886
Application
in vitro, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NOV0281
From
Recombinant Antibody
Specificity
The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e., the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.
Alternative Names
CD32; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; human FCGR2B
UniProt
P31994
Immunogen
The original antibody was isolated from a human combinatorial antibody library by panning against biotinylated human CD32b.
Application Notes
The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 5.4 nM). The binding of the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity 2.4 nM). The antibody bound to CHO cells expressing huCD32b in flow cytometry analysis. The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell- mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell) (US20170198040A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
