Cat. No.
MABL-887
Application
in vitro, in vivo, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NOV1216
From
Recombinant Antibody
Specificity
The antibody specifically binds to CD32b. The antibody mainly recognizes epitope I (i.e. the Fc binding domain area). The antibody does not cross reacts with human CD32a-R.
Alternative Names
CD32; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; human FCGR2B
UniProt
P31994
Immunogen
The original antibody was isolated from a human combinatorial antibody library by panning against biotinylated human CD32b.
Application Notes
The specificity of the antibody for CD32b was determined by ELISA analysis. The antibody was able to bind to CD32b expressed on human B cells by flow cytometry analysis (binding affinity 1.4 nM). The binding the antibody to the BJAB cell line was evaluated by flow cytometry (binding affinity = 2.4 nM). The antibody bound to CHO cells expressing huCD32b by flow cytometry analysis.The human IgG1 format of the antibody was evaluated for its activity in a primary NK cell based antibody-dependent cell- mediated cytotoxicity (ADCC) assay. The antibody demonstrated concentration dependent specific cell lysis of both cancer cell lines evaluated (Jeko-1 and Karpas422 Cancer Cell). The antibody showed a dose dependent in vivo efficacy in mice bearing established Daudi Xenografts. The ability of Fc WT, Fc enhanced and Fc silent versions of the antibody to bind CD32b positive target cells (such as DAUDI, ATCC CCL-213 and Jeko-1, DSMZ ACC533) and subsequently activate CD16a on Jurkat-NFAT v158 reporter cells was evaluated by the Jurkat-NFAT reporter assay. The Fc WT and the Fc enhanced versions yielded to CD16a activation. The Fc Silent version of the antibody enhanced CD16a activation by rituximab and obinutuzumab when CD32b and CD20 are co-expressed on the same target cells. The enhancement is believed to be due to blocking of CD32b binding to the Fc portion of rituximab and obinutuzumab (US20170198040A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
