Cat. No.
MABL-885
Application
Block, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MDE-9
From
Recombinant Antibody
Specificity
This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.
Alternative Names
CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; FcgammaRIIa- H131; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt
P12318
Immunogen
The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
Application Notes
MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).
Antibody First Published
van Royen-Kerkhof et al. Flow cytometric determination of FcgammaRIIa (CD32) polymorphism. J Immunol Methods (2004); 294(1-2):135-44. PMID:15604023
Note on publication
Describes the use of this antibody in the study of FcgammaRIIa (CD32) polymorphism.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
