Cat. No.
MABL-884
Application
Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MDE-8
From
Recombinant Antibody
Specificity
This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.
Alternative Names
CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt
P12318
Immunogen
The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
Application Notes
The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment.Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60% decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30% decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).
Antibody First Published
van Royen-Kerkhof et al. A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice. Br J Haematol. (2005); 130(1):130-7.PMID:15982355
Note on publication
Describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
