Cat. No.
MABL-883
Application
inhibit, IP, Block, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
IV.3
From
Recombinant Antibody
Specificity
This antibody recognizes and binds CD32a.
Alternative Names
CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt
P12318
Immunogen
The original antibody was generated against the FcR of K562 cells.
Application Notes
This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID： 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic “FCGR2A” mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).
Antibody First Published
Looney et al. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J Immunol. (1986); 136(5)：1641-7. PMID：2936805
Note on publication
Describes the fact that U937 cells contain a 40kd FcR which is defined by a monoclonal antibody IV.3.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
