Cat. No.
MABL-879
Application
FACS, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rabbit, Human, Mouse
Clone No.
JC70
From
Recombinant Antibody
Specificity
This antibody recognises CD31 (PECAM1) which is a member of the immunoglobulin superfamily and is expressed at various circulatng cells, such as neutrophils, monocytes, platelets as well as on endothelial cells. CD31 is a cell adhesion molecule and is involved in the leukocyte transendothelial migration during inflammation.
Alternative Names
PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; JC/70A
UniProt
P16284
Immunogen
This antibody was raised by immunising Balb/c mice with membrane preparation from a spleen affected by hairy cell leukaemia.
Application Notes
This antibody recognises CD31 which is widely expressed on endothelial cells (Parums et al., 1990) and JC70 is a good marker of endothelial cells in paraffin sections. It may be used in the identification and characterisation of benign and malignant human vascular disorders (Parums et al., 1990). For instance, one group utilised this antibody to analyse formalin-fixed paraffin-embedded rat heart tissue in a study investigating relation between cisplatin and oxidative stress (Stojic et al., 2018). Similarly, another group labelled pancreatic tumour tissue samples against CD31 while studying factors influencing development of pancreatic cancer-associated thrombosis (Woei-A-Jin et al., 2016). Finally, Kandemir and colleagues (2012) stained formalin-fixed and paraffin-embedded samples of cutaneous Kaposi sarcoma in order to characterise its immunohistochemical profile.
Antibody First Published
Parums et al. JC70：a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol. 1990 Sep;43(9)：752-7. PMID：2212067
Note on publication
This article describes generation and characterisation of JC70 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
