Cat. No.
MABL-839
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Nanobody 52
From
Recombinant Antibody
Specificity
This antibody binds human BAFF protein. B-cell-activating factor (BAFF/CD257) is a cytokine belonging to the tumour-necrosis factor (TNF) family. This cytokine binds to TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF is a survival factor for B cells that controls B-cell maturation. It is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.
Alternative Names
BLyS; TNFSF13B; TALL-1; TNFSF20; ZTNF4; Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; B-cell-activating factor; Dendritic cell-derived TNF-like molecule; TNF and APOL-related leukocyte expressed ligand 1
UniProt
Q9Y275
Immunogen
The original antibody was generated by immunizing an alpaca with extracellular sequence of human BAFF.
Application Notes
This VHH antibody binds human BLyS with a moderate binding affinity of Kd= 3.18E-06M. The inhibitory activity of this activity of this antibody to precent binding of BLyS to its receptors BCMA and TACI was evaluated using ELISA in a competitive inhibition assay. This antibody was also capable of inhibiting BLyS stimulated proliferation of Raji cells (CN104804091).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
