Cat. No.
MABL-803
Application
IB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
NLDC145
From
Recombinant Antibody
Specificity
This antibody is specific for murine DEC-205, a lectin endocytic receptor expressed at high levels by many dendritic cell subsets, where it mediates antigen uptake. The epitope recognised by this antibody is trypsin-sensitive.
Alternative Names
Ly-75; NLDC-145; DEC205; DEC-205; Lymphocyte antigen 75; Ly-75
UniProt
Q60767
Immunogen
This antibody was raised by immunising Wistar rats with mouse lymph node stroma.
Application Notes
This antibody has been used in IHC analysis of acetone-fixed mouse lymph node sections and cytospin preparations (Krall et al, 1986), and immunofluorescence analysis of epidermal sheets ( Krall et al, 1986).It has also been used in immunoblot experiments (Inaba et al, 1995) and in FC experiments to identify DEC-205-expressing cell subsets (Inaba et al, 1995; Hawiger et al, 2001).
Antibody First Published
Kraal et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody J Exp Med. 1986 Apr 1; 163(4)： 981–997 PMID：3950549
Note on publication
Describes the original generation of this antibody, and characterisation of the dendritic cell subsets to which it binds.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
