Cat. No.
MABL-802
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
3B6
From
Recombinant Antibody
Specificity
This antibody recognises chicken CD205 which is an endocytic receptor that is expressed at high levels by cortical thymic epithelial cells and by dendritic cell subsets, including the splenic CD8+ dendritic cell population. Antigen endocytosed via CD205 enters the MHC class I and MHC class II antigen presentation pathways and is subsequently presented to both CD4+ and CD8+ T cells (Shrimpton et al., 2009).
Alternative Names
Lymphocyte antigen 75; LY75; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6
UniProt
Immunogen
This antibody was raised by immunising mice against the recombinant C-type lectin domains 4-6 of the chicken CD205 protein.
Application Notes
3B6 antibody can be used to isolate chicken dendritic cells from peritoneal exudate cells; Vuong and colleagues (2019) did it using magnetic bead cell sorting. This allows performing further studies on this cell type in chicken. Furthermore, this antibody may also be used in the identification of the chicken dendtitic cells via immunofluorescence and cell sorting (Vuong et al., 2019).
Antibody First Published
Vuong et al. CD205-positive, Sepharose-induced peritoneal exudate cells： a new resource for DC research in the chicken. Vet Res Commun. 2019 May;43(2)：115-122. doi： 10.1007/s11259-019-09751-1. Epub 2019 Apr 16. PMID：30989431
Note on publication
This article describes the generation and characterisation of 3B6 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
