Cat. No.
MABL-762
Application
Depletion, FACS, in vivo, IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
FMC63
From
Recombinant Antibody
Specificity
This antibody is specific for human CD19, a 95 kDa protein expressed in the B-lymphocyte lineage.
Alternative Names
B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; Leu12; T-cell surface antigen Leu-12
UniProt
P15391
Immunogen
Human prolymphocytic leukaemia cell line JVM3.
Application Notes
FMC63 was used for four years as a CD19 antibody in various labs prior to its original publication. There, it was reported that the antibody percipitates CD19 and inhibits the binding of other CD19 antibodies to it (Zola et al., 1991; PMID: 1725979). FMC63 was used for IF and FACS of peripheral blood mononuclear cells (PBMC) from patients with multiple myeloma (MM) (Szczepek et al., 1997; PMID: 9057669) and receptor for hyaluronan (HA)-mediated motility (RHAMM) expressing malignant B-lineage cells in myeloma (Crainie et al., 1999; PMID: 10029598). FMC63 was used for IHC and IIP of B cells in lymphocyte-filled villi and IIP of jejunal isolated lymphoid follicle, respectively, in small bowel specimens from human patients (Moghaddami et al., 1998; PMID: 9834269). FNC63 was used to induce B cell depletion in vivo (DiLillo et al., 2011; PMID: 21248259). FMC63 has been incorporated into a CD19 scFv- 41BB- CD3ζ fusion protein-encoding lentiviral vector, which was used in combination with a UCART19 cell therapy to cure high-risk CD19+ infant acute lymphoblastic leukemia (Qasim et al., 2017). This clone did not compete with clone HD37 (available as Ab00214) (Sakemura et al., 2023; PMID: 37879074).
Antibody First Published
Zola et al. Preparation and characterization of a chimeric CD19 monoclonal antibody Immunol Cell Biol. 1991 Dec;69 ( Pt 6):411-22. doi: 10.1038/icb.1991.58 PMID:1725979
Note on publication
The original publication discusses the production of FMC63, a mouse-human chimera of the CD19 cluster monoclonal antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
