Cat. No.
MABL-741
Application
ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Pig (Sus scrofa)
Clone No.
2A10 (2A10/11)
From
Recombinant Antibody
Specificity
This antibody recognizes and binds porcine CD163.
Alternative Names
M130; sCD163; Scavenger receptor cysteine-rich type 1 protein M130
UniProt
Q2VL90
Immunogen
The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
Application Notes
This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID： 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID：23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID：21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID： 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID： 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).
Antibody First Published
Bullido et al. Monoclonal antibodies specific for porcine monocytes/macrophages： macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens (1997); 49(4)：403-13. PMID：9151393
Note on publication
Describes the distribution of surface antigens on porcine monocytes and macrophages using monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
