Anti-CD166 mAbs [EJ212/007-C12-5](MABL-742)

MABL-742

functional assay, IP, WB, ELISA, IHC

Engineer antibody

Human

EJ212/007-C12-5

Recombinant Antibody

This antibody is specific for human CD166. CD166 is a protein found in cancer tissues. CD166 is also cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6.

CD166 antigen; ALCAM; Activated leukocyte cell adhesion molecule

Q13740

This antibody was identified and then reformatted and recombinantly expressed.

The ability of the scFv version of this antibody to bind to PM-1, PBL and granulocytes was assayed by ELISA and flow cytometry. This confirmed that it only bound PM-1 (CA2682059A1).Futhermore the selectivity of the scFv version of this antibody was tested on human tumor tissues including breast, prostate and kidney tumors from 11-14 different donors. breast, prostate and kidney tumors showed the strongest binding. Then to test the specificty of tumors versus normal tissue a tissue microarray with 13 human breast tumor tissues and to a tissue microarray with 4 normal breast tissues using immunohistochemistry staining was preformed with the scFv version of this antibody. 6/13 tumors showed specific staining and none of the normal tissues showed any staining (CA2682059A1). To capture the antigen to which this antibody binds, immunoprecipitation was preformed with the human version of this antibody (CA2682059A1). Futhermore the immuoprecipitated protein was blotted using the human version of this antibody (CA2682059A1). To evaluate the activity of the human version of this antibody as an inductor of apoptosis the effect of the antibody on BT474 was studied. I did indeed induce apoptosis. (CA2682059A1). By detecting the scFv version of this antibody with a secondary antibody it was determined that PM-1 cells showed some internalization of this antibody (CA2682059A1).

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.