Cat. No.
MABL-740
Application
IP, Block, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2.4G2
From
Recombinant Antibody
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse FcγIII and FcγII, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the FcγI receptor (CD64) via its Fc domain.
Alternative Names
Fcγ III/II Receptor; Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII; FcRIII; CD16; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII IgG Fc rec
UniProt
P08508; P08101
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Application Notes
This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.
Antibody First Published
Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3)：580-96. PMID：90108
Note on publication
Describes the generation of rat monoclonal antibodies directed against the mouse FcγIII and FcγII receptors.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
